Bannard, O., Kraman, M., Fearon, D. T. (January 2009) Secondary replicative function of CD8+ T cells that had developed an effector phenotype. Science, 323 (5913). pp. 505-9. ISSN 1095-9203 (Electronic)0036-8075 (Linking)
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Abstract
Models of the differentiation of memory CD8+ T cells that replicate during secondary infections differ over whether such cells had acquired effector function during primary infections. We created a transgenic mouse line that permits mapping of the fate of granzyme B (gzmB)-expressing CD8+ T cells and their progeny by indelibly marking them with enhanced yellow fluorescent protein (EYFP). Virus-specific CD8+ T cells express gzmB within the first 2 days of a primary response to infection with influenza, without impairment of continued primary clonal expansion. On secondary infection, virus-specific CD8+ T cells that became EYFP+ during a primary infection clonally expand as well as all virus-specific CD8+ T cells. Thus, CD8+ T cells that have acquired an effector phenotype during primary infection may function as memory cells with replicative function.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals CD8-Positive T-Lymphocytes/ cytology/ immunology Cell Differentiation Cell Division Granzymes/genetics/metabolism Immunologic Memory Influenza A Virus, H1N1 Subtype/immunology Influenza A Virus, H3N2 Subtype/immunology Luminescent Proteins/genetics/metabolism Lung/immunology Mice Mice, Transgenic Orthomyxoviridae Infections/ immunology Phenotype Spleen/immunology T-Lymphocyte Subsets/cytology/immunology |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells |
| CSHL Authors: | |
| Communities: | CSHL labs > Fearon lab |
| Depositing User: | Matt Covey |
| Date: | 23 January 2009 |
| Date Deposited: | 22 Jul 2015 20:13 |
| Last Modified: | 03 Oct 2019 13:32 |
| PMCID: | PMC2653633 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31649 |
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