Secondary replicative function of CD8+ T cells that had developed an effector phenotype

Bannard, O., Kraman, M., Fearon, D. T. (January 2009) Secondary replicative function of CD8+ T cells that had developed an effector phenotype. Science, 323 (5913). pp. 505-9. ISSN 1095-9203 (Electronic)0036-8075 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/19164749

DOI: 10.1126/science.1166831

Abstract

Models of the differentiation of memory CD8+ T cells that replicate during secondary infections differ over whether such cells had acquired effector function during primary infections. We created a transgenic mouse line that permits mapping of the fate of granzyme B (gzmB)-expressing CD8+ T cells and their progeny by indelibly marking them with enhanced yellow fluorescent protein (EYFP). Virus-specific CD8+ T cells express gzmB within the first 2 days of a primary response to infection with influenza, without impairment of continued primary clonal expansion. On secondary infection, virus-specific CD8+ T cells that became EYFP+ during a primary infection clonally expand as well as all virus-specific CD8+ T cells. Thus, CD8+ T cells that have acquired an effector phenotype during primary infection may function as memory cells with replicative function.

Item Type:	Paper
Uncontrolled Keywords:	Animals CD8-Positive T-Lymphocytes/ cytology/ immunology Cell Differentiation Cell Division Granzymes/genetics/metabolism Immunologic Memory Influenza A Virus, H1N1 Subtype/immunology Influenza A Virus, H3N2 Subtype/immunology Luminescent Proteins/genetics/metabolism Lung/immunology Mice Mice, Transgenic Orthomyxoviridae Infections/ immunology Phenotype Spleen/immunology T-Lymphocyte Subsets/cytology/immunology
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > T cells
CSHL Authors:	Fearon, Douglas
Communities:	CSHL labs > Fearon lab
Depositing User:	Matt Covey
Date:	23 January 2009
Date Deposited:	22 Jul 2015 20:13
Last Modified:	03 Oct 2019 13:32
PMCID:	PMC2653633
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/31649

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