Cre-dependent adeno-associated virus preparation and delivery for labeling neurons in the mouse brain

Huang, Z. J., Taniguchi, H., He, M., Kuhlman, S. (February 2014) Cre-dependent adeno-associated virus preparation and delivery for labeling neurons in the mouse brain. Cold Spring Harb Protoc, 2014 (2). pp. 190-4. ISSN 1559-6095 (Electronic)1559-6095 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/24492777

DOI: 10.1101/pdb.prot080382

Abstract

Virus-mediated gene delivery is a powerful strategy for labeling and manipulating neurons in mammalian brains. A major drawback of this gene delivery method has been the lack of cell-type specificity. However, methods that combine Cre-knockin mice and Cre-activated adeno-associated virus (AAV) have now been developed to achieve high-level, stable, and cell-type-specific gene expression. Here, we describe a protocol for the design, production, and delivery of Cre-dependent AAVs. This method is simple and highly efficient, and allows chronic live imaging of defined classes of synapses in the mouse brain.

Item Type:	Paper
Uncontrolled Keywords:	Animals Brain/ metabolism Dependovirus/classification/ metabolism Gene Transfer Techniques Genetic Vectors/ metabolism Green Fluorescent Proteins/metabolism Integrases/ metabolism Mice Neurons/ metabolism Serotyping
Subjects:	organism description > virus > adenovirus Investigative techniques and equipment > electrophysiology Investigative techniques and equipment > microscopy organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons Investigative techniques and equipment > Whole Brain Circuit Mapping
CSHL Authors:	He, Miao Huang, Z. Josh Kuhlman, Sandra J. Taniguchi, Hiroki
Communities:	CSHL labs > Huang lab
Depositing User:	Matt Covey
Date:	February 2014
Date Deposited:	12 May 2015 20:35
Last Modified:	12 May 2015 20:35
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/31500

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