Cheng, X., Zhang, X., Pflugrath, J. W., Studier, F. W. (April 1994) The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase. Proc Natl Acad Sci U S A, 91 (9). pp. 4034-8. ISSN 0027-8424 (Print)
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Abstract
The lysozyme of bacteriophage T7 is a bifunctional protein that cuts amide bonds in the bacterial cell wall and binds to and inhibits transcription by T7 RNA polymerase. The structure of a mutant T7 lysozyme has been determined by x-ray crystallography and refined at 2.2-A resolution. The protein folds into an alpha/beta-sheet structure that has a prominent cleft. A zinc atom is located in the cleft, bound directly to three amino acids and, through a water molecule, to a fourth. Zinc is required for amidase activity but not for inhibition of T7 RNA polymerase. Alignment of the zinc ligands of T7 lysozyme with those of carboxypeptidase A and thermolysin suggests structural similarity among the catalytic sites for the amidase and these zinc proteases. Mutational analysis identified presumed catalytic residues for amidase activity within the cleft and a surface that appears to be the site of binding to T7 RNA polymerase. Binding of T7 RNA polymerase inhibits amidase activity.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amidohydrolases/metabolism/ ultrastructure Amino Acid Sequence Bacteriophage T7/ enzymology Binding Sites Comparative Study Crystallography, X-Ray DNA-Directed RNA Polymerases/ antagonists & inhibitors Metalloproteins/ultrastructure Molecular Sequence Data Muramidase/chemistry/metabolism/ ultrastructure Protein Structure, Tertiary Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Structure-Activity Relationship Viral Proteins Zinc |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 26 April 1994 |
| Date Deposited: | 24 Jun 2015 20:50 |
| Last Modified: | 09 Nov 2017 19:40 |
| PMCID: | PMC43717 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31466 |
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