Balendiran, K., Bonventre, J., Knott, R., Jack, W., Benner, J., Schildkraut, I., Anderson, J. E. (May 1994) Expression, purification, and crystallization of restriction endonuclease PvuII with DNA containing its recognition site. Proteins, 19 (1). pp. 77-9. ISSN 0887-3585 (Print)
Abstract
We have overexpressed the type II restriction endonuclease PvuII (R-PvuII) in E. coli, prepared large amounts of the homogeneous enzyme, and crystallized it with an oligonucleotide carrying a PvuII recognition site. The cocrystals are orthorhombic space group P2(1)2(1)2(1) with cell constants a = 95.8 A, b = 86.3 A, c = 48.5 A, and diffract X-rays to at least 2.7 A. There is a complex of two protein subunits and one oligonucleotide duplex in the asymmetric unit.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Binding Sites Crystallization Crystallography, X-Ray Deoxyribonucleases, Type II Site-Specific/ chemistry/isolation & purification/metabolism Escherichia coli Gene Expression Molecular Sequence Data Oligodeoxyribonucleotides/ metabolism Recombinant Fusion Proteins/isolation & purification/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme Investigative techniques and equipment > x ray crystallography |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | May 1994 |
| Date Deposited: | 05 Aug 2015 19:45 |
| Last Modified: | 05 Aug 2015 19:45 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31450 |
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