Li, Y., Mak, G., Franza, B. R. (December 1994) In vitro study of functional involvement of Sp1, NF-kappa B/Rel, and AP1 in phorbol 12-myristate 13-acetate-mediated HIV-1 long terminal repeat activation. J Biol Chem, 269 (48). pp. 30616-9. ISSN 0021-9258 (Print)
Abstract
We examined the cooperative activity between the Sp1 and NF-kappa B/Rel sites of the human immunodeficiency virus type 1 long terminal repeat in response to phorbol 12-myristate 13-acetate (PMA) stimulation in an in vitro transcription assay. Sp1 sites alone do not account for the activation induced by PMA. When mutations in Sp1 sites were combined with mutations in the NF-kappa B/Rel sites, a dramatic reduction in PMA-induced transcriptional activity was observed. This reduction was much greater than the reduction associated with mutations involving only the NF-kappa B/Rel sites. This finding suggests that there is functional cooperation between Sp1 and NF-kappa B/Rel and that this is one possible mechanism for transcription activation by NF-kappa B/Rel. The three AP1 sites in the negative regulatory region of the long terminal repeat, however, seem to be uninvolved in the earliest moments of transcriptional activation by PMA.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Binding Sites Cell Nucleus/ metabolism Comparative Study DNA, Viral/genetics/metabolism HIV Long Terminal Repeat/drug effects HIV-1/ genetics Humans Kinetics Molecular Sequence Data Mutagenesis, Site-Directed NF-kappa B/ metabolism Recombinant Proteins/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sp1 Transcription Factor/ metabolism Templates, Genetic Tetradecanoylphorbol Acetate/ pharmacology Transcription Factor AP-1/ metabolism Transcription, Genetic/drug effects |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription diseases & disorders > viral diseases > HIV |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 2 December 1994 |
| Date Deposited: | 30 Jun 2015 16:09 |
| Last Modified: | 30 Jun 2015 16:09 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31433 |
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