Visualizing hippocampal synaptic function by optical detection of Ca2+ entry through the N-methyl-D-aspartate channel

Malinow, R., Otmakhov, N., Blum, K. I., Lisman, J. (August 1994) Visualizing hippocampal synaptic function by optical detection of Ca2+ entry through the N-methyl-D-aspartate channel. Proc Natl Acad Sci U S A, 91 (17). pp. 8170-4.

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Fura-2 and imaging technology were used to detect intracellular Ca2+ changes in CA1 pyramidal cells in hippocampal slices. During focal synaptic stimulation, one or more highly localized regions of Ca2+ elevation (hot spots) were detected in the dendrites. Ca2+ spread from the center of hot spots with properties consistent with diffusion. Several lines of evidence indicate that these hot spots were due to Ca2+ entry through N-methyl-D-aspartate synaptic channels. The spatial and temporal resolution of the method was sufficient to detect the response of single hot spots to single stimuli, thus providing a real-time method for monitoring local synaptic activity. Using this method, we show that synapses on the same dendrite differ in their probability of response and in their facilitation properties.

Item Type: Paper
Uncontrolled Keywords: 2-Amino-5-phosphonovalerate/pharmacology Animals Calcium/*metabolism Calcium Channels/drug effects/*metabolism Dendrites/physiology Fura-2 Hippocampus/*physiology Kinetics Magnesium/pharmacology Microscopy, Fluorescence Probability Pyramidal Cells/*physiology Rats Receptors, N-Methyl-D-Aspartate/drug effects/*metabolism Synapses/*physiology Time Factors
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Matt Covey
Date: 16 August 1994
Date Deposited: 26 Aug 2015 20:53
Last Modified: 09 Nov 2017 19:37
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