Malinow, R., Otmakhov, N., Blum, K. I., Lisman, J. (August 1994) Visualizing hippocampal synaptic function by optical detection of Ca2+ entry through the N-methyl-D-aspartate channel. Proc Natl Acad Sci U S A, 91 (17). pp. 8170-4.
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Abstract
Fura-2 and imaging technology were used to detect intracellular Ca2+ changes in CA1 pyramidal cells in hippocampal slices. During focal synaptic stimulation, one or more highly localized regions of Ca2+ elevation (hot spots) were detected in the dendrites. Ca2+ spread from the center of hot spots with properties consistent with diffusion. Several lines of evidence indicate that these hot spots were due to Ca2+ entry through N-methyl-D-aspartate synaptic channels. The spatial and temporal resolution of the method was sufficient to detect the response of single hot spots to single stimuli, thus providing a real-time method for monitoring local synaptic activity. Using this method, we show that synapses on the same dendrite differ in their probability of response and in their facilitation properties.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 2-Amino-5-phosphonovalerate/pharmacology Animals Calcium/*metabolism Calcium Channels/drug effects/*metabolism Dendrites/physiology Fura-2 Hippocampus/*physiology Kinetics Magnesium/pharmacology Microscopy, Fluorescence Probability Pyramidal Cells/*physiology Rats Receptors, N-Methyl-D-Aspartate/drug effects/*metabolism Synapses/*physiology Time Factors |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus |
| CSHL Authors: | |
| Communities: | CSHL labs > Malinow lab |
| Depositing User: | Matt Covey |
| Date: | 16 August 1994 |
| Date Deposited: | 26 Aug 2015 20:53 |
| Last Modified: | 09 Nov 2017 19:37 |
| PMCID: | PMC44567 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31411 |
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