Kulakauskas, S., Barsomian, J. M., Lubys, A., Roberts, R. J., Wilson, G. G. (May 1994) Organization and sequence of the HpaII restriction-modification system and adjacent genes. Gene, 142 (1). pp. 9-15. ISSN 0378-1119 (Print)
Abstract
We report the organization of the HpaII restriction and modification (R-M) system from Haemophilus parainfluenzae (recognition sequence: 5'...CCGG...3'), the sequence of the gene coding for the HpaII restriction endonuclease, and the sequence of the upstream flanking DNA. The HpaII system comprises two genes, hpaIIM, coding for the methyltransferase (MTase; 358 amino acids (aa), 40.4 kDa: product, Cm5CGG), and hpaIIR, coding for the restriction endonuclease (ENase; 358 aa, 40.9 kDa: product, C'CGG). The genes are adjacent, they have the same orientation, and they occur in the order hpaIIM then hpaIIR. The ENase bears little as sequence similarity to the isoschizomeric R.BsuFI and R.MspI ENases. Upstream of, and partly overlapping hpaIIM is the coding sequence for a 141-aa protein that resembles the very-short-patch-repair endonuclease (Vsr) of Escherichia coli. Upstream of that is the coding sequence for a protein that resembles valyl-tRNA synthetase (ValS).
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Bacterial Deoxyribonuclease HpaII Deoxyribonucleases, Type II Site-Specific/ genetics/metabolism Haemophilus/ enzymology/genetics Molecular Sequence Data Mutation Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Restriction Mapping Sequence Homology, Amino Acid Site-Specific DNA Methyltransferase (Cytosine-Specific)/ genetics/metabolism Temperature |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > methyltransferase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme |
| CSHL Authors: | |
| Communities: | CSHL labs > Roberts lab |
| Depositing User: | Matt Covey |
| Date: | 3 May 1994 |
| Date Deposited: | 27 Aug 2015 20:22 |
| Last Modified: | 27 Aug 2015 20:22 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31395 |
Actions (login required)
 |
Administrator's edit/view item |