Dynamic, mating-induced gene expression changes in female head and brain tissues of Drosophila melanogaster

Dalton, J. E., Kacheria, T. S., Knott, S. R., Lebo, M. S., Nishitani, A., Sanders, L. E., Stirling, E. J., Winbush, A., Arbeitman, M. N. (2010) Dynamic, mating-induced gene expression changes in female head and brain tissues of Drosophila melanogaster. BMC Genomics, 11. p. 541. ISSN 1471-2164

[thumbnail of Paper]
PDF (Paper)
Knott BMC Genomics 2010.pdf - Published Version

Download (403kB) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/20925960
DOI: 10.1186/1471-2164-11-541


BACKGROUND: Drosophila melanogaster females show changes in behavior and physiology after mating that are thought to maximize the number of progeny resulting from the most recent copulation. Sperm and seminal fluid proteins induce post-mating changes in females, however, very little is known about the resulting gene expression changes in female head and central nervous system tissues that contribute to the post-mating response. RESULTS: We determined the temporal gene expression changes in female head tissues 0-2, 24, 48 and 72 hours after mating. Females from each time point had a unique post-mating gene expression response, with 72 hours post-mating having the largest number of genes with significant changes in expression. At most time points, genes expressed in the head fat body that encode products involved in metabolism showed a marked change in expression. Additional analysis of gene expression changes in dissected brain tissues 24 hours post-mating revealed changes in transcript abundance of many genes, notably, the reduced transcript abundance of genes that encode ion channels. CONCLUSIONS: Substantial changes occur in the regulation of many genes in female head tissues after mating, which might underlie aspects of the female post-mating response. These results provide new insights into the physiological and metabolic changes that accompany changes in female behaviors.

Item Type: Paper
Uncontrolled Keywords: Animals Brain/*metabolism Databases, Genetic Dissection Drosophila melanogaster/*genetics Female *Gene Expression Profiling *Gene Expression Regulation Genes, Insect/*genetics Head Male Organ Specificity/genetics *Sexual Behavior, Animal Time Factors
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 2010
Date Deposited: 18 Mar 2015 15:09
Last Modified: 18 Mar 2015 15:09
PMCID: PMC3091690
Related URLs:
URI: https://repository.cshl.edu/id/eprint/31279

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving