Bassett, A. R., Akhtar, A., Barlow, D. P., Bird, A. P., Brockdorff, N., Duboule, D., Ephrussi, A., Ferguson-Smith, A. C., Gingeras, T. R., Haerty, W., Higgs, D. R., Miska, E. A., Ponting, C. P. (August 2014) Considerations when investigating IncRNA function in vivo. Elife, 3. ISSN 2050-084X
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Abstract
Although a small number of the vast array of animal long non-coding RNAs (IncRNAs) have known effects on cellular processes examined in vitro, the extent of their contributions to normal cell processes throughout development, differentiation and disease for the most part remains less clear. Phenotypes arising from deletion of an entire genomic locus cannot be unequivocally attributed either to the loss of the IncRNA per se or to the associated loss of other overlapping DNA regulatory elements. The distinction between cis- or trans-effects is also often problematic. We discuss the advantages and challenges associated with the current techniques for studying the in vivo function of IncRNAs in the light of different models of IncRNA molecular mechanism, and reflect on the design of experiments to mutate IncRNA loci. These considerations should assist in the further investigation of these transcriptional products of the genome.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > long non-coding RNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | Matt Covey |
| Date: | August 2014 |
| Date Deposited: | 03 Oct 2014 14:52 |
| Last Modified: | 03 Nov 2017 18:52 |
| PMCID: | PMC4132285 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30822 |
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