Klimasauskas, S., Roberts, R. J. (May 1995) Disruption of the target G-C base-pair by the HhaI methyltransferase. Gene, 157 (1-2). pp. 163-4. ISSN 0378-1119 (Print)
Abstract
HhaI MTase binds DNA duplexes containing mismatches at the target base position with higher affinity than that observed for the canonical substrate. The stability of these MTase-DNA complexes inversely correlates with the strength of the base pair that is disrupted upon interaction. This finding may offer a general tool to detect other enzymes that flip bases out of the DNA helix.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Composition Base Sequence Comparative Study Cytosine DNA/chemistry/ metabolism Guanine Molecular Sequence Data Site-Specific DNA Methyltransferase (Cytosine-Specific)/ metabolism Substrate Specificity |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > methyltransferase |
| CSHL Authors: | |
| Communities: | CSHL labs > Roberts lab |
| Depositing User: | Jessica Koos |
| Date: | 19 May 1995 |
| Date Deposited: | 12 Aug 2014 14:46 |
| Last Modified: | 12 Aug 2014 14:46 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30610 |
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