Henry, R. W., Sadowski, C. L., Kobayashi, R., Hernandez, N. (April 1995) A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III. Nature, 374 (6523). pp. 653-6. ISSN 0028-0836 (Print)
Abstract
The TATA-box-binding protein TBP exists in the cell complexed with different sets of TBP-associated factors (TAFs). In general, each of these TBP-TAF complexes is dedicated to transcription by a single RNA polymerase. Thus, SL1, TFIID and TFIIIB are required for transcription by polymerases I, II and III, respectively. Here we characterize a fourth TBP-TAF complex called SNAPc. Unlike the other TBP-TAF complexes, SNAPc is implicated in transcription by two types of polymerases; it is required for transcription of both the RNA polymerase II and III small-nuclear RNA genes and binds specifically to the proximal sequence element PSE, a non-TATA-box basal promoter element common to these two types of genes. In addition to TBP, SNAPc is composed of at least three TAFs, SNAP43, SNAP45 and SNAP50. The predicted amino-acid sequence of SNAP43 reveals that it corresponds to a new protein.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Cross Reactions DNA/metabolism DNA-Binding Proteins/genetics/immunology/ metabolism Hela Cells Humans Molecular Sequence Data RNA Polymerase I/ metabolism RNA Polymerase II/ metabolism RNA, Small Nuclear/ genetics/metabolism Recombinant Proteins/metabolism Research Support, U.S. Gov't, P.H.S. TATA Box TATA-Box Binding Protein Transcription Factors/ genetics/immunology/ metabolism Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > design > amino acid design bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > animal > mammal > primates > hominids > human bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Henry lab CSHL labs > Hernandez lab CSHL labs > Kobayashi lab |
| Depositing User: | Jessica Koos |
| Date: | 13 April 1995 |
| Date Deposited: | 11 Aug 2014 15:59 |
| Last Modified: | 11 Aug 2014 15:59 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30603 |
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