LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth

Doseff, A. I., Arndt, K. T. (November 1995) LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth. Genetics, 141 (3). pp. 857-71. ISSN 0016-6731 (Print)



Saccharomyces cerevisiae mutations that cause a requirement for SSD1-v for viability were isolated, yielding one new gene, LAS1, and three previously identified genes, SIT4, BCK1/SLK1, and SMP3. Three of these genes, LAS1, SIT4, and BCK1/SLK1, encode proteins that have roles in bud formation or morphogenesis. LAS1 is essential and loss of LAS1 function causes the cells to arrest as 80% unbudded cells and 20% large budded cells that accumulate many vesicles at the mother-daughter neck. Overexpression of LAS1 results in extra cell surface projections in the mother cell, alterations in actin and SPA2 localization, and the accumulation of electron-dense structures along the periphery of both the mother cell and the bud. The nuclear localization of LAS1 suggests a role of LAS1 for regulating bud formation and morphogenesis via the expression of components that function directly in these processes.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Cell Size Cell Wall/metabolism Fungal Proteins/genetics/ physiology Genes, Fungal Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Morphogenesis Nuclear Proteins/genetics/ physiology Phosphoprotein Phosphatase/genetics/physiology Protein Kinases/genetics/physiology Recombinant Fusion Proteins/biosynthesis Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ cytology/genetics/growth & development Saccharomyces cerevisiae Proteins
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Mitogen-activated protein kinase
CSHL Authors:
Communities: CSHL labs
Depositing User: Jessica Koos
Date: November 1995
Date Deposited: 15 Aug 2014 19:45
Last Modified: 15 Aug 2014 19:45
PMCID: PMC1206850
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