Doseff, A. I., Arndt, K. T. (November 1995) LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth. Genetics, 141 (3). pp. 857-71. ISSN 0016-6731 (Print)
Abstract
Saccharomyces cerevisiae mutations that cause a requirement for SSD1-v for viability were isolated, yielding one new gene, LAS1, and three previously identified genes, SIT4, BCK1/SLK1, and SMP3. Three of these genes, LAS1, SIT4, and BCK1/SLK1, encode proteins that have roles in bud formation or morphogenesis. LAS1 is essential and loss of LAS1 function causes the cells to arrest as 80% unbudded cells and 20% large budded cells that accumulate many vesicles at the mother-daughter neck. Overexpression of LAS1 results in extra cell surface projections in the mother cell, alterations in actin and SPA2 localization, and the accumulation of electron-dense structures along the periphery of both the mother cell and the bud. The nuclear localization of LAS1 suggests a role of LAS1 for regulating bud formation and morphogenesis via the expression of components that function directly in these processes.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Cell Size Cell Wall/metabolism Fungal Proteins/genetics/ physiology Genes, Fungal Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Morphogenesis Nuclear Proteins/genetics/ physiology Phosphoprotein Phosphatase/genetics/physiology Protein Kinases/genetics/physiology Recombinant Fusion Proteins/biosynthesis Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ cytology/genetics/growth & development Saccharomyces cerevisiae Proteins |
| Subjects: | bioinformatics > genomics and proteomics > design > amino acid design organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Mitogen-activated protein kinase |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Jessica Koos |
| Date: | November 1995 |
| Date Deposited: | 15 Aug 2014 19:45 |
| Last Modified: | 15 Aug 2014 19:45 |
| PMCID: | PMC1206850 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30591 |
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