Das, G., Hinkley, C. S., Herr, W. (April 1995) Basal promoter elements as a selective determinant of transcriptional activator function. Nature, 374 (6523). pp. 657-60. ISSN 0028-0836 (Print)
Abstract
In eukaryotes, activation of transcription involves an interplay between activators bound to cis-regulatory elements and factors bound to basal elements near the start site of transcription. The basal elements, for example the TATA box or proximal sequence element (PSE) of small nuclear RNA (snRNA) promoters, nucleate the assembly of basal transcription complexes, components of which interact with activators. Although one basal transcription complex can interact with many activators, it is unclear whether different basal transcription complexes can direct different responses to particular activators. We show here that changing the arrangement of basal elements can alter the response to transcriptional activation domains. Indeed, in the human U6 snRNA promoter, point mutation of either a TATA box or PSE results in diametrically opposed responses to VP16- and Sp1-derived activation domains. These basal elements can even discriminate small changes in an activation domain. Thus the arrangement of basal promoter elements provides a mechanism for differential regulation of transcription.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence DNA/metabolism Fungal Proteins/metabolism Hela Cells Humans Molecular Sequence Data Promoter Regions (Genetics) Proto-Oncogene Proteins c-fos/genetics RNA, Small Nuclear/genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae Proteins TATA Box Trans-Activation (Genetics) Transcription Factors/metabolism |
| Subjects: | bioinformatics > genomics and proteomics > design > amino acid design bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > animal > mammal > primates > hominids > human bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab |
| Depositing User: | Jessica Koos |
| Date: | 13 April 1995 |
| Date Deposited: | 12 Aug 2014 14:12 |
| Last Modified: | 12 Aug 2014 14:12 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30586 |
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