14-3-3 proteins associate with cdc25 phosphatases

Conklin, D. S., Galaktionov, K., Beach, D. (August 1995) 14-3-3 proteins associate with cdc25 phosphatases. Proc Natl Acad Sci U S A, 92 (17). pp. 7892-6. ISSN 0027-8424 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/7644510


The cdc25 phosphatases play key roles in cell cycle progression by activating cyclin-dependent kinases. Two members of the 14-3-3 protein family have been isolated in a yeast two-hybrid screen designed to identify proteins that interact with the human cdc25A and cdc25B phosphatases. Genes encoding the human homolog of the 14-3-3 epsilon protein and the previously described 14-3-3 beta protein have been isolated in this screening. 14-3-3 proteins constitute a family of well-conserved eukaryotic proteins that were originally isolated in mammalian brain preparations and that possess diverse biochemical activities related to signal transduction. We present evidence that indicates that cdc25 and 14-3-3 proteins physically interact both in vitro and in vivo. 14-3-3 protein does not, however, affect the phosphatase activity of cdc25A. Raf-1, which is known to bind 14-3-3 proteins, has recently been shown to associate with cdc25A and to stimulate its phosphatase activity. 14-3-3 protein, however, has no effect on the cdc25A-kinase activity of Raf-1. Instead, 14-3-3 may facilitate the association of cdc25 with Raf-1 in vivo, participating in the linkage between mitogenic signaling and the cell cycle machinery.

Item Type: Paper
Uncontrolled Keywords: 14-3-3 Proteins Amino Acid Sequence Animals Base Sequence Cell Cycle Proteins/isolation & purification/ metabolism Cell Line Cloning, Molecular DNA, Complementary Hela Cells Humans Molecular Sequence Data Phospholipases A/metabolism Phosphoprotein Phosphatase/isolation & purification/ metabolism Protein Biosynthesis Protein-Serine-Threonine Kinases/isolation & purification/metabolism Proteins/isolation & purification/ metabolism Proto-Oncogene Proteins/isolation & purification/metabolism Proto-Oncogene Proteins c-raf Recombinant Fusion Proteins/biosynthesis/isolation & purification/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/metabolism Signal Transduction Spodoptera Transfection Tyrosine 3-Monooxygenase cdc25 Phosphatase
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
bioinformatics > genomics and proteomics > design > protein design
organism description > yeast > Saccharomyces
organism description > animal > insect > Spodoptera
organism description > animal
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning
organism description > animal > mammal > primates > hominids > human
organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction
Investigative techniques and equipment > transfection
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Jessica Koos
Date: 15 August 1995
Date Deposited: 12 Aug 2014 14:40
Last Modified: 09 Nov 2017 19:28
Related URLs:
URI: https://repository.cshl.edu/id/eprint/30583

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