Growth of diseased human muscle in combined cultures with normal mouse embryonic spinal cord

Witkowski, J. A., Dubowitz, V. (October 1975) Growth of diseased human muscle in combined cultures with normal mouse embryonic spinal cord. Journal of the neurological sciences, 26 (2). pp. 203-20. ISSN 0022-510X (Print)0022-510x

DOI: 10.1016/0022-510X(75)90033-7


Normal and diseased human muscle cells have been grown in combined cultures with 12-14 day embryonic mouse spinal cord explants. Nerve endings on myotubes were found by light and scanning electron microscopy, and motor end-plates were identified by a histochemical reaction for acetylcholinesterase. Contracting myotubes, never observed in cultures of human muscle alone, were found in a culture from normal muscle. Histochemical studies demonstrated the presence of strongly and weakly reacting myotubes for both ATPase pH 9.4 and NADH-TR, but could not be related to the development of fibre types. No differences in morphology or histochemical reactions were found between normal and diseased muscle cells in these combined cultures.

Item Type: Paper
Uncontrolled Keywords: Acetylcholinesterase/metabolism Adenosine Triphosphatases/metabolism Adolescent Adult Animals Child Child, Preschool Culture Media *Culture Techniques Female Humans Infant Male Mice Microtubules/ultrastructure Muscles/enzymology/*physiopathology/ultrastructure Muscular Diseases/pathology/*physiopathology Muscular Dystrophies/physiopathology NADH Tetrazolium Reductase/metabolism Spinal Cord/*embryology
Subjects: Investigative techniques and equipment > cell culture
organs, tissues, organelles, cell types and functions > tissues types and functions > myoblast
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: October 1975
Date Deposited: 22 Jul 2014 19:28
Last Modified: 22 Jul 2014 19:28
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