Witkowski, J. A., Dubowitz, V. (October 1975) Growth of diseased human muscle in combined cultures with normal mouse embryonic spinal cord. Journal of the neurological sciences, 26 (2). pp. 203-20. ISSN 0022-510X (Print)0022-510x
Abstract
Normal and diseased human muscle cells have been grown in combined cultures with 12-14 day embryonic mouse spinal cord explants. Nerve endings on myotubes were found by light and scanning electron microscopy, and motor end-plates were identified by a histochemical reaction for acetylcholinesterase. Contracting myotubes, never observed in cultures of human muscle alone, were found in a culture from normal muscle. Histochemical studies demonstrated the presence of strongly and weakly reacting myotubes for both ATPase pH 9.4 and NADH-TR, but could not be related to the development of fibre types. No differences in morphology or histochemical reactions were found between normal and diseased muscle cells in these combined cultures.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Acetylcholinesterase/metabolism Adenosine Triphosphatases/metabolism Adolescent Adult Animals Child Child, Preschool Culture Media *Culture Techniques Female Humans Infant Male Mice Microtubules/ultrastructure Muscles/enzymology/*physiopathology/ultrastructure Muscular Diseases/pathology/*physiopathology Muscular Dystrophies/physiopathology NADH Tetrazolium Reductase/metabolism Spinal Cord/*embryology |
| Subjects: | Investigative techniques and equipment > cell culture organs, tissues, organelles, cell types and functions > tissues types and functions > myoblast |
| CSHL Authors: | |
| Communities: | Banbury Center |
| Depositing User: | Matt Covey |
| Date: | October 1975 |
| Date Deposited: | 22 Jul 2014 19:28 |
| Last Modified: | 22 Jul 2014 19:28 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30470 |
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