Cowan, G. M., Gann, A. A., Murray, N. E. (January 1989) Conservation of complex DNA recognition domains between families of restriction enzymes. Cell, 56 (1). pp. 103-9. ISSN 0092-8674 (Print)0092-8674
Abstract
One polypeptide, designated S, confers sequence-specificity to the multisubunit type I restriction enzymes. Two families of such enzymes, K and A, include members that recognize diverse, bipartite, target sequences. The S polypeptides of the K family, while having areas of near identity, also contain two extensive regions of variable sequence. We now show that one of these, comprising the N-terminal 150 amino acids, specifies recognition of one component of the bipartite target sequence. We have determined the sequence recognized by EcoE, a member of the A family. This sequence, 5'GAG(N7)ATGC, has the trinucleotide GAG in common with EcoA and with StySB of the K family. We determined the nucleotide sequences of the S genes of EcoA and EcoE, and compared their predicted amino acid sequences with each other and with those of the five members of the K family. There is no general sequence similarity between families, but the domain of the S polypeptide of StySB, which specifies GAG, shows nearly 50 per cent identity with the amino variable region of the S polypeptides of EcoA and EcoE. A complex domain that recognizes and directs methylation of GAG is therefore common to enzymes of generally dissimilar amino acid sequence.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence DNA Mutational Analysis DNA-Binding Proteins/*physiology Deoxyribonucleases, Type I Site-Specific/*physiology Escherichia coli/*enzymology Methylation Molecular Sequence Data Substrate Specificity |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme |
| CSHL Authors: | |
| Communities: | School of Biological Sciences > SBS Administration |
| Depositing User: | Matt Covey |
| Date: | 13 January 1989 |
| Date Deposited: | 23 Jun 2014 19:23 |
| Last Modified: | 09 Sep 2021 18:53 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30341 |
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