Modulation of [3H]paroxetine binding to the 5-hydroxytryptamine uptake site in an animal model of depression

Edwards, E., Harkins, K., Wright, G., Henn, F. (May 1991) Modulation of [3H]paroxetine binding to the 5-hydroxytryptamine uptake site in an animal model of depression. Journal of Neurochemistry, 56 (5). pp. 1581-6. ISSN 0022-3042

DOI: 10.1111/j.1471-4159.1991.tb02054.x


The effects of learned helplessness on the 5-hydroxytryptamine (5-HT) uptake site were studied in rats using [3H]paroxetine binding. This ligand was chosen because it was demonstrated to label directly the 5-HT uptake site whereas the [3H]imipramine binding site has been demonstrated to be heterogeneous in nature. Moreover, [3H]imipramine appears to bind to a presynaptic recognition site different from the uptake site. Exposure to uncontrollable shock training and testing resulted in an overall increase in [3H]paroxetine binding in all the groups studied [nonhelpless (NLH), learned helpless (LH), spontaneously helpless (SPLH)] as compared to naive controls (NC). However, the increase in [3H]paroxetine binding was significantly higher in the LH and SPLH groups. The maximum number of [3H]paroxetine binding sites in the rat hippocampus was increased significantly in learned helpless rats (LH and SPLH) at day 4 and day 30 after the shock escape test as compared to NC and NLH rats. By contrast, in the rat hypothalamus the maximum number of [3H]paroxetine binding sites was reduced significantly in the LH rats as compared to naive controls and NLH rats during the same time course. There was no change in [3H]paroxetine binding sites in any other brain regions examined in LH, NLH, and NC rats. The results suggest that a hippocampal hypothalamic connection might play a role in the serotonergic mediation of learned helpless behavior.

Item Type: Paper
Uncontrolled Keywords: Animals Binding Sites Depression/*metabolism Male Paroxetine Piperidines/*metabolism Rats Receptors, Serotonin/*metabolism Reference Values Serotonin Antagonists/metabolism Tritium/diagnostic use
Subjects: diseases & disorders > mental disorders > mood disorders > depression
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neurotransmitter
CSHL Authors:
Communities: CSHL labs > Henn lab
Depositing User: Matt Covey
Date: May 1991
Date Deposited: 09 Jul 2014 14:42
Last Modified: 09 Jul 2014 14:42
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