Functional assessment of GABA uptake or exchange by synaptosomal fractions

Sellstrom, A., Venema, R., Henn, F. (December 1976) Functional assessment of GABA uptake or exchange by synaptosomal fractions. Nature, 264 (5587). pp. 652-3. ISSN 0028-0836 (Print)0028-0836

DOI: 10.1038/264652a0


THE discovery of a transport system in nervous tissue for an amino acid with an apparent K m equal to or less than 10−5 M has been taken as support of a neurotransmitter function for this compound. “High affinity” systems of this sort have been described for γ-amino butyric acid (GABA)1, glutamate2, glycine3, aspartate4 and taurine5. Initially it was hoped that such transmitter transport systems could be used to map the utilisation of a given transmitter in defined anatomical regions. Unfortunately nonspecific elements, especially glial cells, also have the capacity to transport neurotransmitter candidates using “high affinity” systems6. Furthermore, Levi and Raiteri7, noting that “high affinity” transport was found where the internal concentration of the amino acid was high, suggested that isotopic measurements of inward transport were misleading and that exchange of labelled amino acid for unlabelled amino acid is likely to be the phenomena that has been measured8. This called into question the functional role of “high affinity” transport systems, and has resulted in considerable controversy9. In an effort to clarify the conditions of amino acid uptake, we have measured net GABA movement in nerve-terminal-enriched fractions

Item Type: Paper
Uncontrolled Keywords: Aminobutyrates/*metabolism Animals Biological Transport, Active/drug effects Cerebral Cortex/*metabolism/ultrastructure Potassium/pharmacology Rabbits Sodium/pharmacology Synaptosomes/*metabolism gamma-Aminobutyric Acid/*metabolism
Subjects: bioinformatics > genomics and proteomics > small molecules > GABA
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Henn lab
Depositing User: Matt Covey
Date: 16 December 1976
Date Deposited: 09 Jul 2014 19:10
Last Modified: 09 Jul 2014 19:10
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