Associative learning disrupted by impaired Gs signaling in Drosophila mushroom bodies

Connolly, J. B., Roberts, I. J., Armstrong, J. D., Kaiser, K., Forte, M., Tully, T., O'Kane, C. J. (December 1996) Associative learning disrupted by impaired Gs signaling in Drosophila mushroom bodies. Science, 274 (5295). pp. 2104-7. ISSN 0036-8075 (Print)

DOI: 0.1126/science.274.5295.2104


Disruptions in mushroom body (MB) or central complex (CC) brain structures impair Drosophila associative olfactory learning. Perturbations in adenosine 3',5' monophosphate signaling also disrupt learning. To integrate these observations, expression of a constitutively activated stimulatory heterotrimeric guanosine triphosphate-binding protein alpha subunit (Galphas*) was targeted to these brain structures. The ability to associate odors with electroshock was abolished when Galphas* was targeted to MB, but not CC, structures, whereas sensorimotor responses to these stimuli remained normal. Expression of Galphas* did not affect gross MB morphology, and wild-type Galphas expression did not affect learning. Thus, olfactory learning depends on regulated Gs signaling in Drosophila MBs.

Item Type: Paper
Uncontrolled Keywords: Adenylate Cyclase/metabolism Animals Brain/anatomy & histology/metabolism Conditioning (Psychology) Drosophila/ metabolism Electroshock GTP-Binding Protein alpha Subunits, Gs/genetics/ metabolism Guanosine Triphosphate/metabolism Neurons/ metabolism Odors Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Signal Transduction Smell/physiology Transgenes
Subjects: organism description > animal > insect > Drosophila
organism description > animal behavior > learning
organs, tissues, organelles, cell types and functions > tissues types and functions > mushroom body
CSHL Authors:
Communities: CSHL labs > Tully lab
Depositing User: Matt Covey
Date: 20 December 1996
Date Deposited: 21 May 2014 19:58
Last Modified: 21 May 2014 19:58
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