Sadowski, C. L., Henry, R. W., Kobayashi, R., Hernandez, N.
(April 1996)
The SNAP45 subunit of the small nuclear RNA (snRNA) activating protein complex is required for RNA polymerase II and III snRNA gene transcription and interacts with the TATA box binding protein.
Proceedings of the National Academy of Sciences, 93 (9).
pp. 4289-93.
ISSN 0027-8424 (Print)
Abstract
The RNA polymerase II and III small nuclear RNA (snRNA) promoters contain a common basal promoter element, the proximal sequence element (PSE). The PSE binds a multisubunit complex we refer to as the snRNA activating protein complex (SNAPc). At least four polypeptides are visible in purified SNAPc preparations, which migrate with apparent molecular masses of 43, 45, 50, and 190 kDa on SDS/polyacrylamide gels. In addition, purified preparations of SNAPc contain variable amounts of TATA box binding protein (TBP). An important question is whether the PSEs of RNA polymerase II and III snRNA promoters recruit the exact same SNAP complex or slightly different versions of SNAPc, differing, for example, by the presence or absence of a subunit. To address this question, we are isolating cDNAs encoding different subunits of SNAPc. We have previously isolated the cDNA encoding the 43-kDa subunit SNAP43. We now report the isolation of the cDNA that encodes the p45 polypeptide. Antibodies directed against p45 retard the mobility of the SNAPc-PSE complex in an electrophoretic mobility shift assay, indicating that p45 is indeed part of SNAPc. We therefore refer to this protein as SNAP45. SNAP45 is exceptionally proline-rich, interacts strongly with TBP, and, like SNAP43, is required for both RNA polymerase II and III transcription of snRNA genes.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Amino Acid Sequence
Cloning, Molecular
DNA-Binding Proteins/ metabolism
Humans
Macromolecular Substances
Molecular Sequence Data
Molecular Weight
Promoter Regions (Genetics)
RNA Polymerase II/ metabolism
RNA Polymerase III/ metabolism
RNA, Small Nuclear/ biosynthesis
RNA-Binding Proteins/isolation & purification/ metabolism
Recombinant Fusion Proteins/isolation & purification/metabolism
Research Support, U.S. Gov't, P.H.S.
Ribonucleoproteins, Small Nuclear/ metabolism
TATA Box
TATA-Box Binding Protein
Transcription Factors/ metabolism
Transcription, Genetic |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > SNAP protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hernandez lab
CSHL labs > Kobayashi lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
30 April 1996 |
| Date Deposited: |
13 May 2014 15:38 |
| Last Modified: |
13 Sep 2019 16:15 |
| PMCID: |
PMC39528 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30103 |
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