Nestler, H. P., Doseff, A. (August 1997) A two-dimensional, diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique to screen for protease substrates in protein mixtures. Analytical Biochemistry, 251 (1). pp. 122-5. ISSN 0003-2697 (Print)
Abstract
Proteases have emerged during the past few years as key players in the control of cell fate. Many have been identified and cloned, but to understand their function and to elucidate the pathways in which they are involved, the intracellular substrates processed by each protease must be identified. Even the intensive efforts in genome sequencing and cloning that yield new protein sequences every day do not provide a complete solution: the occurrence of a cleavage motif in a protein sequence is not sufficient for proteolytic susceptibility since folding may prevent access of the protease to the substrate site.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Autoradiography Cattle Electrophoresis, Gel, Two-Dimensional/ methods Endopeptidases/ metabolism Evaluation Studies Molecular Weight Proteins/chemistry/ isolation & purification/ metabolism Research Support, Non-U.S. Gov't Sodium Dodecyl Sulfate Substrate Specificity |
| Subjects: | bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease |
| CSHL Authors: | |
| Communities: | CSHL labs > Labeznik lab CSHL labs > Nestler lab |
| Depositing User: | Kathleen Darby |
| Date: | 15 August 1997 |
| Date Deposited: | 07 May 2014 19:06 |
| Last Modified: | 07 May 2014 19:06 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30009 |
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