Photon upmanship: why multiphoton imaging is more than a gimmick

Denk, W., Svoboda, K. (March 1997) Photon upmanship: why multiphoton imaging is more than a gimmick. Neuron, 18 (3). pp. 351-7. ISSN 0896-6273

DOI: 10.1016/S0896-6273(00)81237-4


The unique niche that light microscopy occupies in biology is based on the ability to perform observations on living tissue at relatively high spatial resolution. This resolution is limited by the wavelength of light and does not rival that of electron microscopy, which is, however, a fundamentally nonvital form of observation. Other vital microscopies, such as MRI, can neither resolve subcellular structures nor provide the exquisite molecular selectivity that allows the detection of even single molecules in a background of billions of others.

Item Type: Paper
Uncontrolled Keywords: Brain Chemistry Calcium/analysis Embryo/ultrastructure Equipment Design Forecasting Lasers *Microscopy/instrumentation/methods Microscopy, Confocal Microscopy, Fluorescence Optics *Photons
Subjects: organism description > animal > developmental stage > embryo
Investigative techniques and equipment > optical devices > lasers
Investigative techniques and equipment > microscopy
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: Kathleen Darby
Date: March 1997
Date Deposited: 08 May 2014 17:50
Last Modified: 08 May 2014 17:50
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