Martini, E., Roche, D. M., Marheineke, K., Verreault, A., Almouzni, G. (November 1998) Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells. Journal of Cell Biology, 143 (3). pp. 563-75.
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Abstract
The subcellular distribution and posttranslational modification of human chromatin assembly factor 1 (CAF-1) have been investigated after UV irradiation of HeLa cells. In an asynchronous cell population only a subfraction of the two large CAF-1 subunits, p150 and p60, were found to exist in a chromatin-associated fraction. This fraction is most abundant during S phase in nonirradiated cells and is much reduced in G2 cells. After UV irradiation, the chromatin-associated form of CAF-1 dramatically increased in all cells irrespective of their position in the cell cycle. Such chromatin recruitment resembles that seen for PCNA, a DNA replication and repair factor. The chromatin-associated fraction of p60 was predominantly hypophosphorylated in nonirradiated G2 cells. UV irradiation resulted in the rapid recruitment to chromatin of phosphorylated forms of the p60 subunit. Furthermore, the amount of the p60 and p150 subunits of CAF-1 associated with chromatin was a function of the dose of UV irradiation. Consistent with these in vivo observations, we found that the amount of CAF-1 required to stimulate nucleosome assembly during the repair of UV photoproducts in vitro depended upon both the number of lesions and the phosphorylation state of CAF-1. The recruitment of CAF-1 to chromatin in response to UV irradiation of human cells described here supports a physiological role for CAF-1 in linking chromatin assembly to DNA repair.
| Item Type: | Paper |
|---|---|
| Additional Information: | 0021-9525 (Print) Journal Article Research Support, Non-U.S. Gov't |
| Uncontrolled Keywords: | Animals Cell Fractionation Cell Nucleus/metabolism Chromatin/*metabolism/radiation effects *Chromosomal Proteins, Non-Histone DNA-Binding Proteins/*metabolism/physiology/radiation effects G2 Phase Hela Cells Humans Octoxynol Phosphorylation Proliferating Cell Nuclear Antigen/physiology Rabbits *Ultraviolet Rays |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromatid bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Depositing User: | Kathleen Darby |
| Date: | 2 November 1998 |
| Date Deposited: | 01 May 2014 17:44 |
| Last Modified: | 01 May 2014 17:44 |
| PMCID: | PMC2148138 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29916 |
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