Calcium-evoked dendritic exocytosis in cultured hippocampal neurons. Part I: trans-Golgi network-derived organelles undergo regulated exocytosis

Maletic-Savatic, M., Malinow, R. (September 1998) Calcium-evoked dendritic exocytosis in cultured hippocampal neurons. Part I: trans-Golgi network-derived organelles undergo regulated exocytosis. Journal of Neuroscience, 18 (17). pp. 6803-13. ISSN 0270-6474

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Abstract

Exocytosis is a widely observed cellular mechanism for delivering transmembrane proteins to the cell surface and releasing signaling molecules into the extracellular space. Calcium-evoked exocytosis, traditionally thought to be restricted to presynaptic specializations in neurons, has been described recently in many cells. Here, calcium-evoked dendritic exocytosis (CEDE) is visualized in living cultured hippocampal neurons. Organelles that undergo CEDE are in somata, dendrites, and perisynaptic regions, identified by using immunocytochemistry and correlative light and electron microscopy. CEDE is regulated developmentally: neurons <9 d in vitro do not show CEDE. In addition, CEDE is blocked by tetanus toxin, an inhibitor of regulated exocytosis, and nocodazole, an inhibitor of microtubule polymerization. Organelles that undergo CEDE often are found on the base of spines, putative sites of synaptic plasticity. CEDE therefore could be involved in structural and functional modification of spines and could play a role in synaptic plasticity, where it might involve changes in receptor/channel density, release of active compounds having effect on pre- and postsynaptic function, and/or growth of synaptic structures.

Item Type: Paper
Uncontrolled Keywords: Animals Calcium/ pharmacology Cells, Cultured Dendrites/ drug effects Exocytosis/ drug effects Fluorescent Dyes Golgi Apparatus/ drug effects Hippocampus/cytology/ drug effects Immunohistochemistry Neurons/ drug effects Organelles/drug effects Pyridinium Compounds Quaternary Ammonium Compounds Rats Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > organelles, types and functions
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
CSHL labs > Malinow lab
Depositing User: Kathleen Darby
Date: 1 September 1998
Date Deposited: 01 May 2014 17:04
Last Modified: 01 May 2014 17:04
Related URLs:
URI: https://repository.cshl.edu/id/eprint/29913

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