Futcher, B., Latter, G. I., Monardo, P., McLaughlin, C. S., Garrels, J. I. (November 1999) A sampling of the yeast proteome. Molecular and Cellular Biology, 19 (11). pp. 7357-68. ISSN 0270-7306 (Print)
Abstract
In this study, we examined yeast proteins by two-dimensional (2D) gel electrophoresis and gathered quantitative information from about 1,400 spots. We found that there is an enormous range of protein abundance and, for identified spots, a good correlation between protein abundance, mRNA abundance, and codon bias. For each molecule of well-translated mRNA, there were about 4,000 molecules of protein. The relative abundance of proteins was measured in glucose and ethanol media. Protein turnover was examined and found to be insignificant for abundant proteins. Some phosphoproteins were identified. The behavior of proteins in differential centrifugation experiments was examined. Such experiments with 2D gels can give a global view of the yeast proteome.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Codon Electrophoresis, Gel, Two-Dimensional Fungal Proteins/ isolation & purification Gene Expression Profiling Genes, Fungal Genetic Code Image Processing, Computer-Assisted Protein Biosynthesis RNA, Fungal/ isolation & purification RNA, Messenger/ isolation & purification Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/chemistry/ genetics |
| Subjects: | bioinformatics > genomics and proteomics > analysis and processing > codon processing organism description > yeast > Saccharomyces organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Futcher lab |
| Depositing User: | Kathleen Darby |
| Date: | November 1999 |
| Date Deposited: | 23 Apr 2014 19:36 |
| Last Modified: | 08 May 2014 19:05 |
| PMCID: | PMC84729 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29848 |
Actions (login required)
 |
Administrator's edit/view item |