Kimura, K., Rybenkov, V. V., Crisona, N. J., Hirano, T., Cozzarelli, N. R. (July 1999) 13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensation. Cell, 98 (2). pp. 239-48. ISSN 0092-8674 (Print)
Abstract
Xenopus 13S condensin converts interphase chromatin into mitotic-like chromosomes, and, in the presence of ATP and a type I topoisomerase, introduces (+) supercoils into DNA. The specific production of (+) trefoil knots in the presence of condensin and a type II topoisomerase shows that condensin reconfigures DNA by introducing an ordered, global, (+) writhe. Knotting required ATP hydrolysis and cell cycle-specific phosphorylation of condensin. Condensin bound preferentially to (+) supercoiled DNA in the presence of ATP but not in its absence. Our results suggest a mechanism for the compaction of chromatin by condensin during mitosis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenosine Triphosphate/metabolism Adenosinetriphosphatase/ metabolism Animals Cell Cycle/physiology Chromosomes/chemistry/ enzymology DNA Topoisomerases, Type I/metabolism DNA Topoisomerases, Type II/metabolism DNA, Superhelical/ chemistry/metabolism/ultrastructure DNA-Binding Proteins/ metabolism Hydrolysis Kinetics Microscopy, Electron Mitosis/physiology Multiprotein Complexes Nucleic Acid Conformation Oocytes/physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Xenopus |
| Subjects: | bioinformatics > genomics and proteomics > small molecules > ATP bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function Investigative techniques and equipment > microscopy > electron microscopy organism description > animal > Frog > xenopus |
| CSHL Authors: | |
| Communities: | CSHL labs > Hirano lab |
| Depositing User: | Kathleen Darby |
| Date: | 23 July 1999 |
| Date Deposited: | 28 Apr 2014 14:25 |
| Last Modified: | 28 Apr 2014 14:25 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29832 |
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