Sun, Q., Mayeda, A., Hampson, R. K., Krainer, A. R., Rottman, F. M. (December 1993) General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes & Development, 7 (12b). pp. 2598-608. ISSN 0890-9369 (Print)0890-9369
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Abstract
The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Alternative Splicing/*physiology Animals Cattle Enhancer Elements, Genetic/*physiology Growth Hormone/*genetics HeLa Cells Humans Nuclear Proteins/metabolism/*physiology RNA, Heterogeneous Nuclear/metabolism RNA-Binding Proteins/metabolism/*physiology |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons > exon splicing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Krainer lab |
| Depositing User: | Matt Covey |
| Date: | December 1993 |
| Date Deposited: | 11 Mar 2014 19:36 |
| Last Modified: | 11 Mar 2014 19:36 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29603 |
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