Caceres, J. F., Stamm, S., Helfman, D. M., Krainer, A. R. (September 1994) Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. Science, 265 (5179). pp. 1706-9. ISSN 0036-8075 (Print)0036-8075
Abstract
The opposing effects of SF2/ASF and heterogeneous nuclear ribonucleoprotein (hnRNP) A1 influence alternative splicing in vitro. SF2/ASF or hnRNP A1 complementary DNAs were transiently overexpressed in HeLa cells, and the effect on alternative splicing of several cotransfected reporter genes was measured. Increased expression of SF2/ASF activated proximal 5' splice sites, promoted inclusion of a neuron-specific exon, and prevented abnormal exon skipping. Increased expression of hnRNP A1 activated distal 5' splice sites. Therefore, variations in the intracellular levels of antagonistic splicing factors influence different modes of alternative splicing in vivo and may be a natural mechanism for tissue-specific or developmental regulation of gene expression.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenovirus E1A Proteins/genetics *Alternative Splicing Base Sequence DNA, Complementary/genetics Exons Gene Expression Regulation Genes, Reporter HeLa Cells *Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Humans Molecular Sequence Data Nuclear Proteins/*genetics/*metabolism RNA-Binding Proteins Ribonucleoproteins/genetics/*metabolism Transfection Tropomyosin/genetics |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Alternative Splicing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Krainer lab |
| Depositing User: | Matt Covey |
| Date: | 16 September 1994 |
| Date Deposited: | 11 Mar 2014 19:59 |
| Last Modified: | 11 Mar 2014 19:59 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29595 |
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