Mayeda, A., Krainer, A. R.
(January 1992)
Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2.
Cell, 68 (2).
pp. 365-75.
ISSN 0092-8674 (Print)0092-8674
Abstract
When messenger RNA precursors (pre-mRNAs) containing alternative 5' splice sites are spliced in vitro, the relative concentrations of the heterogeneous ribonucleoprotein (hnRNP) A1 and the essential splicing factor SF2 precisely determine which 5' splice site is selected. In general, an excess of hnRNP A1 favors distal 5' splice sites, whereas an excess of SF2 results in utilization of proximal 5' splice sites. The regulation of these antagonistic activities may play an important role in the tissue-specific and developmental control of gene expression by alternative splicing.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Adenovirus Early Proteins
Base Sequence
Cloning, Molecular
Gene Expression Regulation/*genetics
HeLa Cells
*Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Heterogeneous-Nuclear Ribonucleoproteins
Humans
Immunoblotting
Molecular Sequence Data
Nuclear Proteins/*metabolism
Oncogene Proteins, Viral/genetics
RNA Precursors/genetics/*metabolism
RNA Splicing/genetics/*physiology
RNA-Binding Proteins
Ribonucleoproteins/*metabolism |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Alternative Splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Krainer lab |
| Depositing User: |
Matt Covey
|
| Date: |
24 January 1992 |
| Date Deposited: |
11 Mar 2014 20:48 |
| Last Modified: |
11 Mar 2014 20:48 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29590 |
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