He, B., Chen, P., Chen, S. Y., Vancura, K. L., Michaelis, S., Powers, S. (December 1991) RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins. Proceedings of the National Academy of Sciences of the United States of America, 88 (24). pp. 11373-7. ISSN 0027-8424
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Abstract
In the yeast Saccharomyces cerevisiae, mutations in either of two unlinked genes, RAM1 or RAM2, abolish the farnesyltransferase activity responsible for prenylation of Ras proteins and the a-factor mating pheromone. Here we report that the function of RAM1 and RAM2 genes is required for the membrane localization of Ras proteins and a-factor. The RAM2 gene was sequenced and can encode a 38-kDa protein. We examined the functional interaction of RAM2 and RAM1 by expressing the genes in Escherichia coli. Extracts derived from an E. coli strain that coexpressed RAM1 and RAM2 efficiently farnesylated a-factor peptide and Ras protein substrates. In contrast, extracts derived from E. coli strains that expressed either RAM gene alone were devoid of activity; however, when the latter extracts were mixed, protein farnesyltransferase activity was reconstituted. These results indicate that the yeast farnesyl-protein transferase is comprised of Ram1 and Ram2 polypeptides. Although Ram1 is a component of the enzyme, disruption of the RAM1 gene in yeast was not lethal, indicating that the Ram1-Ram2 farnesyltransferase is not essential for viability. In contrast, disruption of RAM2 was lethal, suggesting that Ram2 has an essential function in addition to its role with Ram1 in protein farnesylation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | *Alkyl and Aryl Transferases Amino Acid Sequence Base Sequence Escherichia coli/genetics Fungal Proteins/*metabolism GTP-Binding Proteins/metabolism *Genes, Fungal Molecular Sequence Data Mutagenesis, Insertional Peptides/*metabolism Pheromones/metabolism Plasmids Restriction Mapping Saccharomyces cerevisiae/enzymology/*genetics Transferases/*genetics/metabolism *ras Proteins |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTP binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Powers lab |
| Depositing User: | Matt Covey |
| Date: | 15 December 1991 |
| Date Deposited: | 26 Feb 2014 15:27 |
| Last Modified: | 30 Sep 2019 14:46 |
| PMCID: | PMC53137 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29517 |
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