Zhu, G. F., Spellman, P. T., Volpe, T., Brown, P. O., Botstein, D., Davis, T. N., Futcher, B. (July 2000) Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth. Nature, 406 (6791). pp. 90-94. ISSN 0028-0836
Abstract
There are about 800 genes in Saccharomyces cerevisiae whose transcription is cell-cycle regulated(1,2). Some of these form clusters of co-regulated genes(1). The 'CLB2' cluster contains 33 genes whose transcription peaks early in mitosis, including CLB1, CLB2, SWI5, ACE2, CDC5, CDC20 and other genes important for mitosis(1). Here we rnd that the genes in this cluster lose their cell cycle regulation in a mutant that lacks two forkhead transcription factors, Fkh1 and Fkh2. Fkh2 protein is associated with the promoters of CLB2, SWI5 and other genes of the cluster. These results indicate that Fkh proteins are transcription factors for the CLB2 cluster. The fkh1 fkh2 mutant also displays aberrant regulation of the 'SIC1' cluster(1), whose member genes are expressed in the M-G1 interval and are involved in mitotic exit. This aberrant regulation may be due to aberrant expression of the transcription factors Swi5 and Ace2, which are members of the CLB2 cluster and controllers of the SIC1 cluster. Thus, a cascade of transcription factors operates late in the cell cycle. Finally, the fkh1 fkh2 mutant displays a constitutive pseudohyphal morphology, indicating that Fkh1 and Fkh2 may help control the switch to this mode of growth.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE FILAMENTOUS GROWTH TRANSCRIPTION SWI5 HEAD EXPRESSION PROTEIN KINASE MCM1 CHITINASE |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Futcher lab |
| Depositing User: | Matt Covey |
| Date: | July 2000 |
| Date Deposited: | 28 Jan 2014 21:55 |
| Last Modified: | 28 Jan 2014 21:55 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29456 |
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