Wilson, A. C., Boutros, M., Johnson, K. M., Herr, W. (September 2000) HCF-1 amino- and carboxy-terminal subunit association through two separate sets of interaction modules: Involvement of fibronectin type 3 repeats. Molecular and Cellular Biology, 20 (18). pp. 6721-6730. ISSN 0270-7306
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Abstract
When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (BCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result from an unusual proteolytic processing of a large precursor polypeptide. Here, we have characterized the structure and function of sequences required for HCF-1(N) and HCF-1(C) subunit association. HCF-1 contains two matched pairs of self-association sequences called SAS1 and SAS2. One of these matched association sequences, SAS1, consists of a short 43-amino-acid region of the HCF-1(N) subunit, which associates with a carboxy-terminal region of the HCF-1(C) subunit that is composed of a tandem pair of fibronectin type 3 repeats, a structural motif known to promote protein-protein interactions. Unexpectedly, the related protein HCF-2, which is not proteolyzed, also contains a functional SAS1 association element, suggesting that this element does not function solely to maintain HCF-1(N) and HCF-1(C) subunit association. HCF-1(N) subunits do not possess a nuclear localization signal. We show that, owing to a carboxy-terminal HCF-1 nuclear localization signal, HCF-1(C) subunits can recruit HCF-1(N) subunits to the nucleus.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | HERPES-SIMPLEX VIRUS HOST-CELL FACTOR CRYSTAL-STRUCTURE NUCLEAR-LOCALIZATION PROTEIN HCF C1 FACTOR VP16 DOMAINS COMPLEX FAMILY |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification organism description > virus > herpes simplex virus organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab |
| Depositing User: | Matt Covey |
| Date: | September 2000 |
| Date Deposited: | 31 Jan 2014 14:36 |
| Last Modified: | 31 Jan 2014 14:36 |
| PMCID: | PMC86190 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29396 |
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