Willy, P. J., Kobayashi, R., Kadonaga, J. T. (November 2000) A basal transcription factor that activates or represses transcription. Science, 290 (5493). pp. 982-984. ISSN 0036-8075
Abstract
We have identified an activity that is required for transcription of downstream promoter element (DPE)-containing core promoters in vitro. The purified factor was found to be the Drosophila homolog of the transcriptional repressor known as NC2 or Dr1-Drap1. Purified recombinant dNC2 activates DPE-driven promoters and represses TATA-driven promoters. A mutant version of dNC2 can activate DPE promoters but is unable to repress TATA promoters. Thus, the activation and repression functions are distinct. These studies reveal that NC2 (Dr1-Drap1) is a bifunctional basal transcription factor that differentially regulates gene transcription through DPE or TATA box motifs.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | II GENE-TRANSCRIPTION CORE PROMOTER ELEMENT NEGATIVE COFACTOR IN-VIVO BINDING NC2 DROSOPHILA MECHANISM TFIIA DR1 |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Kobayashi lab |
| Depositing User: | Matt Covey |
| Date: | November 2000 |
| Date Deposited: | 31 Jan 2014 16:21 |
| Last Modified: | 31 Jan 2014 16:21 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29355 |
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