Wysocka, J., Liu, Y., Kobayashi, R., Herr, W. (May 2001) Developmental and cell-cycle regulation of Caenorhabditis elegans HCF phosphorylation. Biochemistry, 40 (19). pp. 5786-5794. ISSN 0006-2960
Abstract
HCF-1 is a mammalian protein required for cell proliferation. It is also involved in transcriptional activation of herpes-simplex-virus immediate-early gene transcription in association with the viral transactivator VP16. HCF-1 and a related protein called HCF-2 possess a homologue in Caenorhabditis elegans that can associate with and activate VP16. Here, we demonstrate developmental regulation of C. elegans HCF (CeHCF) phosphorylation: a hyperphosphorylated form of CeHCF is present in embryos, whereas a hypophosphorylated form is present in L1 larvae. The phosphorylation patterns of endogenous CeHCF in worms and ectopically synthesized CeHCF in mammalian cells are remarkably similar, suggesting that the way CeHCF can be recognized by kinases is conserved in animals. Phosphorylation-site mapping of endogenous CeHCF, however, revealed that phosphorylation occurs at four clustered sites in the region of the protein that is not highly conserved among HCF proteins and is not required for VP16-induced complex formation. Indeed, phosphorylation of either CeHCF or human HCF-1 appears to be dispensable for association with VP16. All four CeHCF phosphorylation sites match the consensus recognition site for the cell-cycle kinases CDC2 and CDK2. Consistent with this similarity and with the developmental phosphorylation of CeHCF in C. elegans embryos, CeHCF phosphorylation is cell-cycle-regulated in mammalian cells.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | OCT-1 HOMEODOMAIN PROTEIN HCF VP16 COMPLEX KINASE FAMILY POLYPEPTIDES PROGRESSION P34(CDC2) DOMAIN |
| Subjects: | organism description > animal > C elegans organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell regulation organism description > animal > developmental stage bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab CSHL labs > Kobayashi lab |
| Depositing User: | Matt Covey |
| Date: | May 2001 |
| Date Deposited: | 15 Jan 2014 20:29 |
| Last Modified: | 15 Jan 2014 20:29 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29332 |
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