Stebbins, M. J., Urlinger, S., Byrne, G., Bello, B., Hillen, W., Yin, J. C. P. (September 2001) Tetracycline-inducible systems for Drosophila. Proceedings of the National Academy of Sciences of the United States of America, 98 (19). pp. 10775-10780. ISSN 0027-8424
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Abstract
Since their inception, tetracycline (Tet)-inducible systems have become the method of choice for transgenic research. The Tet-Off systems have a number of advantages, including robust target induction using a relatively benign effector molecule. However, use of the Tet-On system has been fraught with difficulties, including high background expression in the absence of effector molecules and inconsistent gene induction. Recently, second-generation Tet-On transactivators (TAs) have been described. In HeLa cells, they are far more efficient than the original reverse TA protein, and they exhibit lower background activity in the absence of effectors. Here we examine the most promising TA in transgenic Drosophila and characterize its in vivo properties. We report that low levels of doxycycline, when added to normal fly food, efficiently and rapidly induce target transgenes in adults, larvae, and embryos. This TA is superior to all other Tet-On proteins, and its performance is comparable to that of the widely used Tet-Off TA. In addition, combining the improved Tet-On TA with the Gal4-UAS (upstream-activating sequence) system produces robust, spatially restricted, temporally controlled transgene induction. Because this Tet-On TA is significantly more efficient than previous ones used in Drosophila, it is also possible to modulate gene induction by controlling the dosage of the antibiotic in the food.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | GENE-EXPRESSION CELLS BOUNDARIES ELEMENTS MEMORY ASSAY SCS |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter > TET responsive promoter |
| CSHL Authors: | |
| Communities: | CSHL labs > Yin lab |
| Depositing User: | Matt Covey |
| Date: | September 2001 |
| Date Deposited: | 16 Jan 2014 17:01 |
| Last Modified: | 10 Sep 2019 19:09 |
| PMCID: | PMC58551 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29305 |
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