Gari, E., Volpe, T., Wang, H. Y., Gallego, C., Futcher, B., Aldea, M. (November 2001) Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast. Genes & Development, 15 (21). pp. 2803-2808. ISSN 0890-9369
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Abstract
Eukaryotic cells commit in G(1) to a new mitotic cycle or to diverse differentiation processes. Here we show that Whi3 is a negative regulator of Cln3, a G(1) cyclin that promotes transcription of many genes to trigger the G(1)/S transition in budding yeast. Whi3 contains an RNA-recognition motif that specifically binds the CLN3 mRNA, with no obvious effects on Cln3 levels, and localizes the CLN3 mRNA into discrete cytoplasmic foci. This is the first indication that G(1) events may be regulated by locally restricting the synthesis of a cyclin. Moreover, Whi3 is also required for restraining Cln3 function in meiosis, filamentation, and mating, thus playing a key role in cell fate determination in budding yeast.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | cell cycle G(1) regulation differentiation RNA-binding protein cyclin mRNA localization CEREVISIAE G1 CYCLINS SACCHAROMYCES-CEREVISIAE MESSENGER-RNA LOCALIZATION GROWTH KINASE TRANSCRIPTION FIBROBLASTS INHIBITION EXPRESSION |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Futcher lab |
| Depositing User: | Matt Covey |
| Date: | November 2001 |
| Date Deposited: | 22 Jan 2014 17:43 |
| Last Modified: | 22 Jan 2014 17:43 |
| PMCID: | PMC312816 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29235 |
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