Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain

Peunova, N., Scheinker, V., Cline, H., Enikolopov, G. (November 2001) Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain. Journal of Neuroscience, 21 (22). pp. 8809-8818. ISSN 0270-6474

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11698593

Abstract

Mechanisms controlling the transition of a neural precursor cell from proliferation to differentiation during brain development determine the distinct anatomical features of the brain. Nitric oxide (NO) may mediate such a transition, because it can suppress DNA synthesis and cell proliferation. We cloned the gene encoding the neuronal isoform of Xenopus NO synthase (XNOS) and found that in the developing brain of Xenopus tadpoles, a zone of XNOS-expressing cells lies adjacent to the zone of dividing neuronal precursors. Exogenous NO, supplied to the tadpole brain in vivo, decreased the number of proliferating cells and the total number of cells in the optic tectum. Conversely, inhibition of NOS activity in vivo increased the number of proliferating cells and the total number of cells in the optic tectum. NOS inhibition yielded larger brains with grossly perturbed organization. Our results indicate that NO is an essential negative regulator of neuronal precursor proliferation during vertebrate brain development.

Item Type:	Paper
Uncontrolled Keywords:	nitric oxide Xenopus differentiation proliferation brain optic tectum neuron neuronal precursors SMOOTH-MUSCLE CELLS GROWTH-FACTOR NEURONAL DIFFERENTIATION RIBONUCLEOTIDE REDUCTASE DROSOPHILA DEVELOPMENT NO SYNTHASE PC12 CELLS IN-VITRO EXPRESSION PATHWAY
Subjects:	organs, tissues, organelles, cell types and functions > organs types and functions > brain organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell regulation bioinformatics > genomics and proteomics > small molecules > nitric oxide organism description > animal > Frog > xenopus
CSHL Authors:	Cline, Hollis T. Enikolopov, Grigori N. Peunova, Natalia Scheinker, Vladimir
Communities:	CSHL labs > Cline lab CSHL labs > Enikopolov lab
Depositing User:	Matt Covey
Date:	November 2001
Date Deposited:	20 Dec 2013 15:37
Last Modified:	20 Dec 2013 15:37
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/29133

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