Wu, Q., Krainer, A. R.
(November 1996)
U1-mediated exon definition interactions between AT-AC and GT-AG introns.
Science, 274 (5289).
pp. 1005-1008.
ISSN 0036-8075
Abstract
A minor class of metazoan introns has well-conserved splice sites with 5'-AU-AC-3' boundaries, compared to the 5'-GU-AG-3' boundaries and degenerate splice sites of conventional introns. Splicing of the AT-AC intron 2 of a sodium channel (SCN4A) precursor messenger RNA in vitro did not require inhibition of conventional splicing and required adenosine triphosphate, magnesium, and U12 small nuclear RNA (snRNA). When exon 3 was followed by the 5' splice site from the downstream conventional intron, splicing of intron 2 was greatly stimulated. This effect was U1 snRNA-dependent, unlike the basal AT-AC splicing reaction. Therefore, U1-mediated exon definition interactions can coordinate the activities of major and minor spliceosomes.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
pre-messenger-rna
small nuclear ribonucleoproteins
splice-site
selection
cartilage matrix protein
sodium-channel gene
genomic
structure
last nucleotides
u1 snrnp
invitro
binding |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > introns
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Krainer lab |
| Depositing User: |
Matt Covey
|
| Date: |
November 1996 |
| Date Deposited: |
10 Dec 2013 21:47 |
| Last Modified: |
10 Dec 2013 21:47 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28943 |
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