Distinct functions of the closely related tandem RNA-recognition motifs of hnRNP A1

Mayeda, A., Munroe, S. H., Xu, R. M., Krainer, A. R. (September 1998) Distinct functions of the closely related tandem RNA-recognition motifs of hnRNP A1. RNA, 4 (9). pp. 1111-1123. ISSN 1355-8382

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Abstract

hnRNP A1 regulates alternative splicing by antagonizing SR proteins. It consists of two closely related, tandem RNA-recognition motifs (RRMs), followed by a glycine-rich domain. Analysis of variant proteins with duplications, deletions, or swaps of the RRMs showed that although both RRMs ave required for alternative splicing function, each RRM plays distinct roles, and their relative position is important. Surprisingly, RRM2 but not RRM1 could support this function when duplicated, despite their very similar structure. Specific RNA binding and annealing are not sufficient for hnRNP Al alternative splicing function. These observations, together with phylogenetic and structural data, suggest that the two RRMs are quasi-symmetric but functionally nonequivalent modules that evolved as components of a single bipartite domain.

Item Type:	Paper
Uncontrolled Keywords:	alternative splicing hnRNP A1 RNA binding RNA-recognition motif (RRM) RNA-RNA annealing heterogeneous nuclear ribonucleoprotein splice-site selection poly(a) binding-protein messenger-rna in-vivo crystal-structure complex protein-a1 factor sf2/asf cdna cloning domains
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:	Krainer, Adrian R. Mayeda, Akila Xu, Rui-Ming
Communities:	CSHL labs > Krainer lab CSHL labs > Xu lab
Depositing User:	Matt Covey
Date:	September 1998
Date Deposited:	11 Dec 2013 15:10
Last Modified:	11 Dec 2013 15:10
PMCID:	PMC1369686
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28933

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