Purine-rich enhancers function in the AT-AC pre-mRNA splicing pathway and do so independently of intact U1 snRNP

Wu, Q., Krainer, A. R. (December 1998) Purine-rich enhancers function in the AT-AC pre-mRNA splicing pathway and do so independently of intact U1 snRNP. RNA, 4 (12). pp. 1664-1673. ISSN 1355-8382

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URL: http://www.ncbi.nlm.nih.gov/pubmed/9848661

DOI: 10.1017/s1355838298981432

Abstract

A rare class of introns in higher eukaryotes is processed by the recently discovered AT-AC spliceosome. AT-AC introns are processed inefficiently in vitro, but the reaction is stimulated by exon-definition interactions involving binding of U1 snRNP to the 5' splice site of the downstream conventional intron. We report that purine-rich exonic splicing enhancers also strongly stimulate sodium channel AT-AC splicing. Intact U2, U4, or U6 snRNAs are not required for enhancer function or for exon definition. Enhancer function is independent of U1 snRNP, showing that: splicing stimulation by a downstream 5' splice site and by an exonic enhancer differ mechanistically.

Item Type:	Paper
Uncontrolled Keywords:	AT-AC intron exon definition exonic splicing enhancer (ESE) pre-mRNA splicing U1 snRNP small nuclear ribonucleoprotein immunodeficiency-virus type-1 protein-protein interactions messenger-rna introns sr-proteins in-vivo exon recognition site selection sequences binding
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons > exon splicing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:	Krainer, Adrian R. Wu, Caizhi Wu, Qiang
Communities:	CSHL labs > Krainer lab
Depositing User:	Matt Covey
Date:	December 1998
Date Deposited:	11 Dec 2013 15:05
Last Modified:	11 Dec 2013 15:05
PMCID:	PMC1369733
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28932

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