Caputi, M., Mayeda, A., Krainer, A. R., Zahler, A. M. (July 1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO Journal, 18 (14). pp. 4060-4067. ISSN 0261-4189
Abstract
Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are necessary for HIV-1 splicing inhibition. We demonstrate that heterogeneous nuclear ribonucleoproteins (hnRNPs) of the A and B group are trans-acting factors required for the function of the tnt exon 2 ESS, Depletion of hnRNP A/B proteins from HeLa cell nuclear extract activates splicing of mt exon 2 pre-mRNA substrate. Splicing inhibition is restored by addition of recombinant hnRNP A/B proteins to the depleted extract. A high-affinity hnRNP A1-binding sequence can substitute functionally for the ESS in tnt exon 2, These results demonstrate that hnRNP A/B proteins are required for repression of HIV-1 splicing.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | HIV-1 hnRNP proteins pre-mRNA splicing immunodeficiency-virus type-1 messenger-rna binding-proteins sr proteins tat exon-2 a1 expression silencer selection elements |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification diseases & disorders > viral diseases > HIV bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Krainer lab |
| Depositing User: | Matt Covey |
| Date: | July 1999 |
| Date Deposited: | 11 Dec 2013 19:31 |
| Last Modified: | 11 Dec 2013 19:31 |
| PMCID: | PMC1171481 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28930 |
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