Mayeda, A., Badolato, J., Kobayashi, R., Zhang, M. Q., Gardiner, E. M., Krainer, A. R. (August 1999) Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing. EMBO Journal, 18 (16). pp. 4560-4570. ISSN 0261-4189
Abstract
Biochemical purification of a pre-mRNA splicing activity from HeLa cells that stimulates distal alternative 3' splice sites in a concentration-dependent manner resulted in the identification of RNPS1, a novel general activator of pre-mRNA splicing. RNPS1 cDNAs, encoding a putative nucleic-acid-binding protein of unknown function, were previously identified in mouse and human. RNPS1 is conserved in metazoans and has an RNA-recognition moth preceded by an extensive serine-rich domain. Recombinant human RNPS1 expressed in baculovirus functionally synergizes with SR proteins and strongly activates splicing of both constitutively and alternatively spliced pre-mRNAs, We conclude that RNPS1 is not only a potential regulator of alternative splicing but may also play a more fundamental role as a general activator of pre-mRNA splicing.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | alternative splicing RNA-recognition motif RNPS1 splicing activator splicing factor site selection in-vivo rna protein expression binding factor-sf2 sequence domains transcript |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing |
| CSHL Authors: | |
| Communities: | CSHL labs > Kobayashi lab CSHL labs > Krainer lab CSHL labs > Zhang lab |
| Depositing User: | Matt Covey |
| Date: | August 1999 |
| Date Deposited: | 11 Dec 2013 17:37 |
| Last Modified: | 11 Dec 2013 17:37 |
| PMCID: | PMC1171530 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28924 |
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