Nonaka, N., Kitajima, T., Yokobayashi, S., Xiao, G. P., Yamamoto, M., Grewal, S. I. S., Watanabe, Y. (January 2002) Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast. Nature Cell Biology, 4 (1). pp. 89-93. ISSN 1465-7392
Abstract
Fission yeast centromeres, like those of higher eukaryotes, are composed of repeated DNA structures and associated heterochromatin protein complexes, that have a critical function in the faithful segregation of chromosomes during cell division1, 2, 3. Cohesin protein complexes, which are essential for sister-chromatid cohesion and proper chromosome segregation, are enriched at centromeric repeats4, 5. We have identified a functional and physical link between heterochromatin and cohesin. We find that the preferential localization of cohesins at the centromeric repeats is dependent on Swi6, a conserved heterochromatin protein that is required for proper kinetochore function. Cohesin is also enriched at the mating-type heterochromatic region in a manner that depends on Swi6 and is required to preserve the genomic integrity of this locus. We provide evidence that a cohesin subunit Psc3 interacts with Swi6 and its mouse homologue HP1. These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting the proper segregation of chromosomes.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Grewal lab |
| Depositing User: | Matt Covey |
| Date: | January 2002 |
| Date Deposited: | 08 Jan 2014 14:55 |
| Last Modified: | 08 Jan 2014 14:55 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28758 |
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