Modification of position-effect variegation by competition for binding to Drosophila satellites

Monod, C., Aulner, N., Cuvier, O., Kas, E. (August 2002) Modification of position-effect variegation by competition for binding to Drosophila satellites. Embo Reports, 3 (8). pp. 747-752. ISSN 1469-221X

URL: http://www.ncbi.nlm.nih.gov/pubmed/12151333

DOI: 10.1093/embo-reports/kvf155

Abstract

white-mottled (wm4) position-effect variegation (PEV) arises by translocation of the white gene near the pericentric AT-rich 1.688 g/cm3 satellite III (SATIII) repeats of the X chromosome of Drosophila. The natural and artificial A•T-hook proteins D1 and MATH20 modify wm4 PEV in opposite ways. D1 binds SATIII repeats and enhances PEV, presumably via a recruitment of protein partners, whereas MATH20 suppresses it. We show that D1 and MATH20 compete for binding to identical sites of SATIII repeats in vitro and that conditional MATH20 expression results in a displacement of D1 from pericentric heterochromatin in vivo. In the presence of intermediate levels of MATH20, we show that this displacement becomes selective for SATIII repeats. These results strongly suggest that the suppression of wm4 PEV by MATH20 is due to a displacement of D1 from its preferred binding sites and provide additional support for a direct role of D1 in the assembly of AT-rich heterochromatin.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA translocation organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
CSHL Authors:	Cuvier, Olivier
Communities:	CSHL labs > Hirano lab
Depositing User:	Matt Covey
Date:	August 2002
Date Deposited:	07 Jan 2014 21:32
Last Modified:	07 Jan 2014 21:32
PMCID:	PMC1084209
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/28751

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