Bazett-Jones, D. P., Kimura, K., Hirano, T. (June 2002) Efficient supercoiling of DNA by a single condensin complex as revealed by electron spectroscopic imaging. Molecular Cell, 9 (6). pp. 1183-1190. ISSN 1097-2765
Abstract
Condensin, a five-subunit protein complex essential for mitotic chromosome condensation from yeast to humans, introduces positive supercoils into DNA in an ATP-dependent manner in vitro. We report here the direct visualization of this supercoiling reaction by electron spectroscopic imaging. In the presence of ATP, a single condensin complex is capable of introducing two or more compensatory supercoils into the protein-free region of a closed circular DNA. Within the condensin-bound region, ∼190 bp of DNA is organized into a compact structure with two distinct domains, indicative of the formation of two oriented gyres. The current results suggest that the action of condensin is more dynamic and more efficient than that postulated before, providing fundamental insight into the energy-dependent mechanism of higher order chromatin folding.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing Investigative techniques and equipment Investigative techniques and equipment > spectroscopy |
| CSHL Authors: | |
| Communities: | CSHL labs > Hirano lab |
| Depositing User: | Matt Covey |
| Date: | June 2002 |
| Date Deposited: | 09 Jan 2014 15:30 |
| Last Modified: | 09 Jan 2014 15:30 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28669 |
Actions (login required)
 |
Administrator's edit/view item |