Makino, H., Li, B. (2013) Monitoring synaptic plasticity by imaging AMPA receptor content and dynamics on dendritic spines. Methods Mol Biol, 1018. pp. 269-275. ISSN 1940-6029 (Electronic)1064-3745 (Linking)
Abstract
Time-lapse imaging techniques are widely used to monitor dendritic spine dynamics, a measurement of synaptic plasticity. However, it is challenging to follow the dynamics of spines over an extended period in vivo during development or in deep brain structures that are beyond the reach of traditional microscopes. Here, we describe an AMPA receptor-based optical approach to monitor recent history of synaptic plasticity. This method allows the identification of spines that have recently acquired synaptic AMPA receptors in a single imaging session, so that synaptic plasticity that occurs in vivo in a variety of conditions can be simply imaged in an ex vivo preparation. © 2013 Springer Science+Business Media, LLC.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | AMPA receptor trafficking Dendritic spine Fluorescence recovery after photobleaching Super Ecliptic pHluorin Synaptic plasticity Two-photon imaging |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines |
| CSHL Authors: | |
| Communities: | CSHL labs > Li lab |
| Depositing User: | Matt Covey |
| Date: | 2013 |
| Date Deposited: | 25 Sep 2013 15:45 |
| Last Modified: | 27 Jan 2017 20:50 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28627 |
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